High Capacity Streptavidin Agarose Resin

Our High Capacity Streptavidin Agarose Beads consist of purified streptavidin covalently conjugated to crosslinked 4% agarose beads. Developed using a proprietary coupling procedure, the biotin-binding activity of the immobilized streptavidin is optimally preserved while minimizing ligand leaching and non-specific binding.

This premium chromatography resin is ideal for the rapid, high-yield isolation and separation of biotinylated targets, including recombinant proteins, nucleic acids, antibodies, and small ligands.

Key Features & Specifications:

• High Binding Capacity: Binds approximately 30 µg of free biotin per mL of settled resin.
• Optimal Performance: Low non-specific binding and leak-resistant streptavidin immobilization across diverse affinity chromatography conditions.
• Versatile Workflows: Perfectly suited for gravity flow columns, spin columns, and batch purification protocols.
• Formulation: Supplied as a 50% suspension in 0.1 M sodium phosphate (pH 7.2), 0.15 M NaCl, and 0.02% sodium azide (2 mL of slurry equals 1 mL of settled resin).

Applications:

• Affinity purification of biotin-tagged proteins and nucleic acid complexes.
• Isolation of Strep-tagged recombinant proteins.
• Solid-phase binding assays and transcription factor pulldowns (e.g., biotinylated DNA probes).
• Immunoprecipitation and antigen purification utilizing biotinylated antibodies.

Storage & Shipping:

Stable for approximately two years when stored at 2–8°C. DO NOT FREEZE, as freezing damages the agarose bead structure. This product requires international shipping on ice packs. Please contact [email protected] for shipping estimates.