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Amersham(TM) Protran® 0.45 µm NC, disks, Ø 25 mm,, 100 sheet(s)Amersham(TM) Protran 0. 45 m NC, disks, 25 mm,, Flash point (flp) 13 C Material: nitrocellulose (100 %). Transfer membrane for most nucleic acid and protein immobilising techniques Directions for use Nitrocellulose should only conditionally be used for electrophoretic transfer of nucleic acids, because of the high salt concentrations required for the transfer. Membranes of 0,45 m pore size allow for DNA transfer (minimum 300 bp) and protein transfer
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Amersham(TM) Protran® 0.45 µm NC, disks, Ø 25 mm,, Flash point (flp) 13 °C

Material: nitrocellulose (100 %).

Transfer membrane for most nucleic acid and protein immobilising techniques

Directions for use

Nitrocellulose should only conditionally be used for electrophoretic transfer of nucleic acids, because of the high salt concentrations required for the transfer. Membranes of 0,45 μm pore size allow for DNA transfer (minimum 300 bp) and protein transfer (minimum 20 kDa).

Application examples

Southern, Northern, Western transfer, colony and plaque hybridisation, immunoblots, nucleic acid and protein dot-blot, slot-blot, electrophoretic transfer of proteins.Detection systems are radioactivity, chemoluminescence, colour

For colony and plaque lifting in small petri dishes.

Technical Information
Dimensions Ø2.5 cm 
Version disks 
Pore size 0,45 µm
Type Protran® NC 0,45 
Bond electrostatic, hydrophobic 
Immobilisation UV, baking (max. 80 °C) 
Binding capacity 86-110 µg/cm²

Amersham(TM) Protran® 0.45 µm NC, disks, Ø 25 mm,, 100 sheet(s)

Item no : 55654602812
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