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home > p-Nitrotetrazolium blue chloride, min. 98 %, p.a., 100 mg, glass > p-Nitrotetrazolium blue chloride, min. 98 %, p.a., 100 mg, glass

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p-Nitrotetrazolium blue chloride, min. 98 %, p.a., 100 mg, glassp Nitrotetrazolium blue chloride, min. 98 %, p. a., Molar mass (M) 817,65 g mol, Storage temp. +4 C, CAS No. 298 83 9, EC No. 206 067 4, Empirical formula C40H30Cl2N10O6 Used in combination with BCIP toluidine salt (X phosphate) for detection of alkaline phosphatase activity by producing an indigo dye precipitate. Colour enhancer for staining with alkalic phosphatase. Mechanism During dimerisation of ketonized X Phos, H+ ions are set free which reduce

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p-Nitrotetrazolium blue chloride, min. 98 %, p.a., Molar mass (M) 817,65 g/mol, Storage temp. +4 °C, CAS No. 298-83-9, EC No. 206-067-4, Empirical formula C₄₀H₃₀Cl₂N₁₀O₆

Used in combination with BCIP-toluidine salt (X-phosphate) for detection of alkaline phosphatase activity by producing an indigo dye precipitate. Colour enhancer for staining with alkalic phosphatase.

Mechanism

During dimerisation of ketonized X-Phos, H⁺ ions are set free which reduce NBT to purple-coloured diformazan. Both dyes precipitate in the direct vicinity of the phosphatase, hence staining this site in a dark violet. Store at +4 °C. Please also see assay protocol for use of BCIP/NBT in immunoblot procedures.

Technical Information

Application	Western, in situ, Histochemistry
Enzyme	Alkaline phosphatase
Detection	Colour (brown-violet)
Signal product	Precipitate (Blot)

Technical Documentation (PDF)

p-Nitrotetrazolium blue chloride, min. 98 %, p.a., 100 mg, glass

Name: p-Nitrotetrazolium blue chloride, min. 98 %, p.a., 100 mg, glass
Brand: okunadegoodman.com
SKU: 79808700199
Price: 24.20 USD
Availability: InStock
Rating: 4.8388 (852 reviews)