APC Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90066-50, BS90066-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P25054(Human) Q61315(Mouse) P70478(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC IHC FC

All Applications: WB:1:500
ICC:1:50-1:200
IHC:1:100-1:200
FC:1:50-1:100

Background: The adenomatous polyposis syndromes, familial adenomatous polyposis (FAP) and Gardner's syndrome (GS), are characterized by numerous adenomatous polyps throughout the entire colon. These polyps invariably progress to colon cancer in addition to other extracolonic manifestations. The cloning of the APC gene revealed a ubiquitously expressed protein, 2,843 amino acids in length, which is frequently mutated in patients suffering from FAP and GS. APC has been found to be associated with structural components of intracellular junctions. β-catenin and γ-catenin (also called plakoglobin), are involved in the regulation of cellular adhesion. APC and E-cadherin compete for binding to specific internal regions of both β- and γ-catenin. Interactions between cytoskeleton and the APC, E-cadherin, β/γ catenin complex are mediated by α-catenin.

Purification and Purity: Peptide affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: APC polyclonal antibody detects endogenous levels of APC protein.

Bioworld Molecular Weight: Predicted band size 312 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: ICC staining APC in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS., ICC staining APC in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: APC, DP2.5, Adenomatous Polyposis Coli, Adenomatous polyposis coli protein, Apc, APC, CC1, Deleted in polyposis 2.5, DP2, DP2.5, DP3, FAP, FPC, GS, Protein APC,

Immunogen: Peptide

Conjugate: Unconjugated

Modification: Unmodified