Cleaved-Caspase-4/5 p20 (D270/D311) rabbit pAb

Sizes: 50μL, 100μL

Catalogue Numbers: ES7702-50, ES7702-100

Citations, Manuals and MSDS Available upon request.

Background: This gene encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain and a large and small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This caspase is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. When overexpressed, this gene induces cell apoptosis. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008],

Alternate Name: CASP4; ICH2; Caspase-4; CASP-4; ICE (rel)-II; Protease ICH-2; Protease TX; CASP5; ICH3; Caspase-5; CASP-5; ICE (rel)-III; Protease ICH-3; Protease TY

Source: Rabbit

Applications: WB; ELISA

Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.

Reactivity: Human

Immunogen: The antiserum was produced against synthesized peptide derived from human Caspase 4/5. AA range:221-270

Storage and Stability: -20°C/1 year

Clonality: Polyclonal

Isotype: IgG

Concentration: 1 mg/ml

Observed Band (KD): 47, 22kD

Human Gene ID: 837

Human SWISS Prot NO: P49662/P51878

Subcellular Location: Cytoplasm, cytosol. Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Mitochondrion. Inflammasome. Secreted. Predominantly localizes to the endoplasmic reticulum (ER). Association with the ER membrane requires TMEM214 (PubMed:15123740). Released in the extracellular milieu by keratinocytes following UVB irradiation (PubMed:22246630).

Research Use Only