ERβ (E101) Polyclonal Antibody

Catalogue Numbers: AP0436-50, AP0436-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q92731

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ERα and ERβ, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. ERα and ERβ have been shown to be differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ERα and ERβ may be regulated by distinct mechanisms even though they share many functional characteristics.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: ERβ (E101) polyclonal antibody detects endogenous levels of ERβ protein.

Extra Notes: Western blot (WB) analysis of ERβ (E101) polyclonal antibody at 1:500 dillution
Lane1:A549 whole cell lysate(40μg)
Lane2:MCF-7 whole cell lysate(40μg)
Lane3:LO2 whole cell lysate(40μg)

Bioworld Molecular Weight: ~ 48, 60 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Estrogen receptor beta; ER-beta; Nuclear receptor subfamily 3 group A member 2; ESR2; ESTRB; NR3A2; ER-β

Immunogen: Synthetic peptide, corresponding to amino acids 71-120 of Human ERβ.

Conjugate: Unconjugated

Modification: Unmodified