CtBP2 (P432) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS2287-50, BS2287-100

Sizes: 50µl, 100µl

Swiss-Prot: P56545

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

Background: CtBP1 and the related protein CtBP2 are characterized as C-terminal binding proteins of adenovirus E1A, and they preferentially associate with the E1A via a 5 amino acid motif, PLDLS, to repress E1A induced oncogenesis and cellular transformation.CtBP1 is expressed from embryo to adult, but CtBP2 is mainly expressed during embryogenesis. During skeletal and T cell development, CtBP1 and CtBP2 associate with the PLDLSL domain of deltaEF1, a cellular zinc finger-homeodomainprotein, and thereby enhances deltaEF1-induced transcriptional silencing. In addition, CtBP complexes with CtIP, a 125 kDa protein that recognizes distinctly different protein motifs from CtBP. CtIP binds to the BRCT repeats within the breast cancer gene BRCA1 and enables CtBP to influence BRCA1 activity.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: CtBP2 (P432) polyclonal antibody detects endogenous levels of CtBP2 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CtBP2 (P432) polyclonal antibody at 1:500 dilution
Lane 1: PC3 whole cell lysate (40ug)
Lane 2: H1792 whole cell lysate (40ug)
Lane 3: Panc1 whole cell lysate (40ug)
Lane 4: H9C2 whole cell lysate (40ug)
Lane 5: AML-12 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of CtBP2 (P432) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~48.0 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: C-terminal-binding protein 2; CtBP2; CTBP2

Immunogen: Synthetic peptide, corresponding to amino acids 400-450 of Human CtBP2.

Conjugate: Unconjugated

Modification: Unmodified