Recombinant Human Fibronectin/FN Protein (Active)

Size: 50μg

Catalogue Number: PKSH032450-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: Fibronectin; FN

Target Symptom: Fibronectin; FN1; CIG; ED-B; FINC; FN; FNZ; GFND; GFND2; LETS; MSF

Research Area: Signal Transduction; Cardiovascular; Cancer; Developmental Biology; Stem cells

Target Species: Human

Expression Host: E.coli

Application: Cell culture

Fusion Tag: None

UNIProt ID: P02751

Accession: P02751

Background: Fibronectin1(FN1) is a secreted protein and contains 12 fibronectin type-I domains; fibronectin type-II domains and 16 fibronectin type-III domains.Recombinant human fibronectin fragment; is a protein of ~63 kDa containing a central cell-binding domain; a high affinity heparin-binding domain II; and CS1 site within the alternatively spliced III CS region of human fibronectin. Cells bind to a VLA-4 ligand; a CS-I site; and a VLA-5 ligand; a cell attachment domain; and virus vectors binds to a heparin binding domain II; which co-locates the cell and the virus vector on NovoNectin. This process enhances the density of both cells and vectors; and facilitates the gene transduction in the result.

Activity: Measured by its ability to support cell attachment and spreading when used as a substratum for cell culture. The ED50 for this effect is 0.1-0.5 ug/ml.

Sequence: Pro1270-Ser1546&Ala1721-Thr2016

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of 12.5 mM Sodium Citrate, 1.25% Sucrose, pH 6.2.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 62.7 kDa

Observed MW: 60 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only