Histone H2B Monoclonal Antibody

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Catalogue Numbers: MB65937-50, MB65937-100

Sizes: 50µl, 100µl

Swiss-Prot: P33778

Host: Mouse

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification and Purity: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Recognizes endogenous levels of Histone H2B protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Histone H2B expression in Hela (A), NIH3T3 (B), Raw264.7 (C), rat brain (D), rat kidney (E) whole cell lysates., Immunohistochemical analysis of Histone H2B staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Bioworld Molecular Weight: ~ 14 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: H2BFF; Histone H2B type 1-B; Histone H2B.1; Histone H2B.f; H2B/f

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human Histone H2B. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified