Recombinant Human RSK3/RPS6KA2 Protein (GST Tag)(Active)

Size: 50μg

Catalogue Number: PKSH030377-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: RSK3; RPS6KA2

Target Symptom: HU-2; MAPKAPK1C; p90-RSK3; pp90RSK3; RSK; RSK3; S6K-alpha; S6K-alpha2

Target Species: Human

Expression Host: Baculovirus-Insect Cells

Application: Enzyme

Fusion Tag: N-GST

UNIProt ID: Q15349

Accession: Q15349-1

Background: Ribosomal protein S6 kinase alpha-2, also known as 90 kDa ribosomal protein S6 kinase 2, MAP kinase-activated protein kinase 1c, MAPK-activated protein kinase 1c, Ribosomal S6 kinase 3, RSK-3, RPS6KA2 and MAPKAPK1C, is a nucleus protein which belongs to theprotein kinase superfamily, AGC Ser/Thr protein kinase family and S6 kinase subfamily. RPS6KA2 / RSK-3 is expressed in many tissues. Highest expression is in lung and skeletal muscle. The expression of RPS6KA2 reduced proliferation, caused G1 arrest, increased apoptosis, reduced levels of phosphorylated extracellular signal-regulated kinase and altered other cell cycle proteins. RPS6KA2 / RSK-3 contains oneAGC-kinase C-terminal domain and twoprotein kinase domains. It forms a complex with either ERK1 or ERK2 in quiescent cells. It transiently dissociates following mitogenic stimulation. RPS6KA2 / RSK-3 is a serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB. RPS6KA1, RPS6KA2, RPS6KB1, RPS6KB2, and PDK1 are involved in several pathways central to the carcinogenic process, including regulation of cell growth, insulin, and inflammation.

Activity: The specific activity was determined to be 41 nmol/min/mg using synthetic RSK peptide (KRRRLSSLRA) as substrate.

Sequence: Met 1-Leu 733

Purity: > 84 % as determined by reducing SDS-PAGE.

Formulation: Supplied as sterile solution of 20mM Tris, 500mM NaCl, pH 7.0, 20mM GSH
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 110 kDa

Observed MW: 110 kDa

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Research Use Only