The E. coli strain ER2738 isÊan amber suppressor (glnV) F⁺ strain with a rapid growth rate and is particularly well-suited for M13 phage propagation. Amid BiosciencesÕ high efficiency chemically competent ER2738 cellsÊcan be used forÊphage display,Êantibody phage displayÊlibrary constructionÊand screening. The F-factor of ER2738 contains a mini-transposon which confers tetracycline resistance, so cells can be selected by plating and propagating in tetracycline-containing medium. ER2738 cultures for infection can be grown either in LB or LB+Tet media. Tetracycline does not need to be added to media during phage amplification. Loss of F-factor in nonselective media is insignificant as long as cultures are not serially diluted. Although ER2738 is a recA+ strain, there are no reports on spontaneous in vivo recombination events with M13 or phagemid vectors.

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