Amid Biosciences offers a specialized Cyclophilin A (CypA) - KRAS G12V Protein Kit designed to accelerate the discovery and validation of novel small-molecule inhibitors targeting oncogenic RAS mutations. This kit provides a complete set of premium reagents to study the formation of target-chaperone binary complexes and downstream drug-mediated tri-complexes, bypassing the classic "undruggable" challenges of smooth RAS surface topography.

The Tri-Complex & Molecular Glue Mechanism

Modern oncology frameworks utilize molecular glues to force structural interactions between active, GTP-bound mutant RAS proteins and abundant cellular chaperones like Cyclophilin A (CypA). This highly selective binary complex creates a novel, composite binding surface that sterically blocks downstream effector interactions (such as RAF binding), effectively shutting down hyperactivated MAPK signaling pathways in mutant RAS cancers.

Kit Components & Inclusions

Each kit contains components sufficient for robust in vitro biochemical screening and biophysical characterization:

• Recombinant Biotinylated Human KRAS G12V (1-185): 0.05 mg | Featuring a specific G12V oncogenic mutation on the switch-sense domain of the isoform KRAS4B.
• Recombinant Biotinylated Human Wild-Type KRAS (1-185): 0.05 mg | Included for critical counter-screening and selectivity profiling.
• Recombinant Human Cyclophilin A (CypA) (1-165): 0.1 mg | Tagged with an N-terminal 6XHis tag for versatile assay orientation and capture.

Validated Assay Applications

This multi-protein system is meticulously formulated and optimized for high-throughput screening (HTS) and binding kinetics, including:

• Surface Plasmon Resonance (SPR) & Bio-Layer Interferometry (BLI)
• AlphaLISA / HTRF proximity configurations
• Targeted protein degradation (TPD) profiling and complex stability assays

Product Specifications

• Expression Host: All components are expressed and purified from optimized Escherichia coli (E. coli) systems.
• Purity: >90% by Coomassie blue staining on SDS-PAGE.
• Storage & Handling: Shipped on dry ice. Strict storage is required at -80°C. To maintain enzymatic and binding integrity, minimize freeze/thaw cycles and snap-freeze remaining protein in liquid nitrogen after initial aliquotting.

Catalog Number: KR12VCP-301 | This product is intended for laboratory research use only (RUO).

Scientific References

1. Schulze CJ et al. Chemical remodeling of a cellular chaperone to target the active state of mutant KRAS. Science. 2023;381(6659):794-799.
2. Deng J, Shen S, Huang L et al. Small-molecule degraders for oncogenic KRAS G12C and pan-KRAS mutations. Nat Commun (2026).