VAV1 is a prominent guanine nucleotide exchange factor (GEF) for the Rho family of GTPases, serving as an essential intracellular signal transducer in hematopoietic cells. It plays a critical role in T-cell receptor (TCR) and B-cell receptor (BCR) signaling, driving T-cell activation, T-helper cell differentiation, actin polymerization, and cytoskeleton reorganization through both GEF-dependent and GEF-independent pathways. Given its central role in mediating adaptive immunity and its implications in tumorigenesis, VAV1 is a high-priority therapeutic target for autoimmune diseases, chronic inflammatory disorders, and T-cell malignancies.

Product Features & Specifications

• Expression System: Engineered and expressed in a high-yield E. coli bacterial system.
• Construct Details: Recombinant human VAV1 fragment spanning amino acids 178–574 (UniProt ID: P15498-1).
• Fusion Tags: Outfitted with an N-terminal 6xHis tag and an AviTag™ peptide sequence for robust purification and oriented immobilization.
• Precision Biotinylation: Site-specifically biotinylated at the N-terminal AviTag™ using BirA enzyme, preserving the native structural conformation of downstream GTPase interaction domains.
• Molecular Mass: Resolves at approximately ~49.7 kDa under reducing conditions on SDS-PAGE.
• Purity Profile: Verified at >85% purity by Coomassie blue staining.
• Formulation & Storage: Supplied at a concentration of 1.0 mg/mL in 20 mM Tris-HCl, pH 7.5, 100 mM NaCl, 5 mM 2-mercaptoethanol, 0.04% Tween 20, and 20% Glycerol. Store securely at -80°C.

Validated Assay Applications

This site-specifically biotinylated human VAV1 construct is optimized for functional target-binding assays, high-throughput screening (HTS) of small molecule inhibitors, Rho GTPase interaction studies, Surface Plasmon Resonance (SPR), Bio-Layer Interferometry (BLI), and streptavidin-coated plate ELISA formats.

Catalog Number: VAV1-B-301 | This product is intended for laboratory research use only (RUO). International shipping requires dry ice packing.